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BrdU (IIB5) Antibody
BrdU (IIB5) Antibody from Santa Cruz Biotechnology, Inc.
Be the first to write a review!
Citations:
(248)
Supplier Page
Supplier Page from
Santa Cruz Biotechnology, Inc. for
BrdU (IIB5) Antibody
Santa Cruz Biotechnology, Inc.'s
BrdU (IIB5) Antibody
is a Mouse monoclonal antibody. This antibody has been shown to work in applications such as: Flow Cytometry, Immunofluorescence, and Immunohistochemistry - fixed.
Description
BrdU (IIB5)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BrdU (IIB5) Antibody
Company
Santa Cruz Biotechnology, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
sc-32323
Antibody Type
Primary Antibodies
Clone
IIB5
Quantity
200 µg/ml
Applications
IF, IHC(P), FCM
Host
mouse
Conjugate/Tag
Unconjugated
Clonality
Monoclonal
Format
Purified
Target
Bromodeoxyuridine (BrdU)
Reactivity
NSR
Isotype
IgG1
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Santa Cruz Biotechnology, Inc.
10410 Finnell Street
Dallas
,
Texas
75220
United States
Phone
: 1-800-457-3801
831-457-3801
Company Profile
Website:
http://www.scbt.com/
Citations (248)
(1)
TDP1 suppresses chromosomal translocations and cell death induced by abortive TOP1 activity during gene transcription
Nature Communications
November 9, 2023
Diana Rubio-Contreras, Fernando Gómez-Herreros
overnight. DNA was denatured with 2 N HCl/
Triton X-100
. Cells were incubated with anti-BrdU (
Santa Cruz
,
sc-32323
) at
1:1000
overnight at 4 °C. After that, AlexaFluor-conjugated secondary antibody (Invitrogen) was
(2)
Long non-coding RNA H19 promotes proliferation in hepatocellular carcinoma cells via H19/miR-107/CDK6 axis
Oncology Research
September 15, 2023
ARCHITTAPON NOKKEAW, PANNATHON THAMJAMRASSRI, NAPHAT CHANTARAVISOOT, PISIT TANGKIJVANICH, CHAIYABOOT ARIYACHET
hydrochloric acid prior to detection
by immunofluorescence
with an anti-BrdU antibody (
1:200
dilution; Cat #
sc-32323
;
Santa Cruz
, Dallas, TX, United States), followed by
incubation
with Alexa Fluor 488-conjugated secondary
(3)
The ndrg2 Gene Regulates Hair Cell Morphogenesis and Auditory Function during Zebrafish Development
International journal of molecular sciences
June 11, 2023
Wang C, Wang X, Zheng H, Yao J, Xiang Y, Liu D.
dilution,
Abcam
, #
ab97959
) and a
mouse
monoclonal anti-BrdU (
1:500
dilution,
Santa Cruz Biotechnology
, #
sc-32323
, Dallas, TX, USA) were used to label the HCs, supporting cells and proliferative cells, respectively.
(4)
Infection-induced extracellular vesicles evoke neuronal transcriptional and epigenetic changes
Scientific reports
April 27, 2023
Tedford E, Badya NB, Laing C, Asaoka N, Kaneko S, Filippi BM, McConkey GA.
immunoprecipitation
was performed with protein G Dynabeads and IgG anti-BrdU antibody IIB5 (
Santa Cruz Biotechnology
,
SC-32323
). RNA was extracted and
RT-qPCR
performed as previously described. Methylation sensitive restriction
(5)
TERRA stability is regulated by RALY and polyadenylation in a telomere-specific manner
Cell reports
April 25, 2023
Savoca V, Rivosecchi J, Gaiatto A, Rossi A, Mosca R, Gialdini I, Zubovic L, Tebaldi T, Macchi P, Cusanelli E.
(6)
FAM193A is a positive regulator of p53 activity
Cell reports
March 28, 2023
Szwarc MM, Guarnieri AL, Joshi M, Duc HN, Laird MC, Pandey A, Khanal S, Dohm E, Bui AK, Sullivan KD,
et al
.
RRID:AB_10695870 BID,Cell Signaling Technology,Cat#2002; RRID:AB_10692485 BrdU,
Santa Cruz Biotechnology
,Cat#
sc-32323
; RRID:AB_626766 Cleaved
Caspase-3
,
Cell Signaling Technology
,Cat#
9661
; RRID:AB_2341188 Caspase-8,
Cell Signaling Technology
(7)
Reelin regulates the migration of late-born hippocampal CA1 neurons via cofilin phosphorylation
Molecular and cellular neurosciences
March 1, 2023
Ishii K, Kohno T, Sakai K, Hattori M.
(8)
Hindering NAT8L expression in hepatocellular carcinoma increases cytosolic aspartate delivery that fosters pentose phosphate pathway and purine biosynthesis promoting cell proliferation
Redox biology
February 1, 2023
De Falco P, Lazzarino G, Felice F, Desideri E, Castelli S, Salvatori I, Ciccarone F, Ciriolo MR.
cell counting
. For BrdU incorporation
assay
, cells were incubated with 10 μM BrdU (
Santa Cruz Biotechnology
,
sc-32323
) for 4 h and fixed for 30 min with 90% ethanol, 5% acetic acid, 5% water fix solution.
DNA denaturation
(9)
Adipose Tissue-Derived Extracellular Vesicles Contribute to Phenotypic Plasticity of Prostate Cancer Cells
International journal of molecular sciences
January 8, 2023
Mathiesen A, Haynes B, Huyck R, Brown M, Dobrian A.
(10)
Alleviation of osteoarthritis by intra-articular transplantation of circulating mesenchymal stem cells
Biochemical and biophysical research communications
December 25, 2022
Lin W, Yang Z, Shi L, Wang H, Pan Q, Zhang X, Zhang P, Lin S, Li G.
(11)
Keratin-mediated hair growth and its underlying biological mechanism
Communications biology
November 19, 2022
An SY, Kim HS, Kim SY, Van SY, Kim HJ, Lee JH, Han SW, Kwon IK, Lee CK, Do SH,
et al
.
staining
was done by
immunocytochemical
staining
with mouse anti-BrdU antibody (
Santa Cruz Biotechnology
,
sc-32323
, diluted
1:100
). In addition, BrdU incorporation
assay
was done by BrdU Cell Proliferation ELISA Kit
(12)
The androgen receptor couples promoter recruitment of RNA processing factors to regulation of alternative polyadenylation at the 3' end of transcripts
Nucleic acids research
September 23, 2022
Caggiano C, Pieraccioli M, Pitolli C, Babini G, Zheng D, Tian B, Bielli P, Sette C.
RNasin ribonuclease
inhibitors
(N2518, Promega), and incubated with 1.25 μg of anti-BrdU antibody (
sc-32323
,
Santa Cruz Biotechnology
) in the presence of Dynabeads protein G (Invitrogen) at 4°C for 2 h. The bead–antibody–RNA
(13)
Protocol for affordable and efficient profiling of nascent RNAs in bread wheat using GRO-seq
STAR protocols
September 16, 2022
Chen Y, Zhu J, Xie Y, Li Z, Zhang Y, Liu M, Dong Z.
RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, BrdU (IIB5):
mouse
monoclonal IgG (3:100),
Santa Cruz Biotechnology
,
sc-32323
,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,
(14)
Circular EZH2-encoded EZH2-92aa mediates immune evasion in glioblastoma via inhibition of surface NKG2D ligands
Nature communications
August 15, 2022
Zhong J, Yang X, Chen J, He K, Gao X, Wu X, Zhang M, Zhou H, Xiao F, An L,
et al
.
kit (AM1907,
Thermo Fisher Scientific
). The NRO-RNA was incubated with anti-
BrdU
antibody (2 μg/tube,
SC-32323
,
clone
IIB5,
Santa Cruz
)-coated Protein G beads for 30 min at room temperature. The beads were captured
(15)
A lncRNA fine-tunes salicylic acid biosynthesis to balance plant immunity and growth
Cell host & microbe
August 10, 2022
Liu N, Xu Y, Li Q, Cao Y, Yang D, Liu S, Wang X, Mi Y, Liu Y, Ding C,
et al
.
(16)
GREM1/PPP2R3A expression in heterogeneous fibroblasts initiates pulmonary fibrosis
Cell & bioscience
August 6, 2022
Shi X, Wang J, Zhang X, Yang S, Luo W, Wang S, Huang J, Chen M, Cheng Y, Chao J.
with
goat
serum at room temperature for 2 h. The cells were incubated with a BrdU antibody (
1:100
;
SC-32323
,
Santa Cruz
) at 4 °C overnight. After washing with PBS, the cells were incubated with appropriate fluorescent
(17)
IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism
Redox biology
August 1, 2022
Li Y, Yang S, Liu Y, Qin L, Yang S.
kits and 2-Deoxy-d-glucose (2-DG) were ordered from
Sigma
. Antibodies against
MMP13
(
sc-515284
), BrdU (
sc-32323
), ColX (
sc-59954
), actin (
sc-47778
), Lamin (
sc-377000
) and Smad2/3 (
sc-133098
) were obtained from
Santa Cruz Biotechnology
(18)
Phosphorylation of mixed lineage kinase MLK3 by cyclin-dependent kinases CDK1 and CDK2 controls ovarian cancer cell division
The Journal of biological chemistry
August 1, 2022
Cedeno-Rosario L, Honda D, Sunderland AM, Lewandowski MD, Taylor WR, Chadee DN.
(19)
Trp53 controls chondrogenesis and endochondral ossification by negative regulation of TAZ activity and stability via β-TrCP-mediated ubiquitination
Cell death discovery
July 12, 2022
Li Y, Yang ST, Yang S.
insulin-transferrin-sodium selenite media supplement (ITS Supplement) were purchased from
Cell Signaling Technology
. BrdU (#
sc-32323
; dilution
1:1000
), β-TrCP (#
sc-390629
; dilution
1:1000
), GFP (#
sc-9996
; dilution
1:1000
) and flag (#
sc-7945
;
(20)
WDR62 variants contribute to congenital heart disease by inhibiting cardiomyocyte proliferation
Clinical and translational medicine
July 1, 2022
Hao L, Ma J, Wu F, Ma X, Qian M, Sheng W, Yan T, Tang N, Jiang X, Zhang B,
et al
.
embryos were incubated with the rabbit anti‐Histone H3 (phospho S10) (
ab5176
,
Abcam
) and mouse anti‐BrdU (
sc‐32323
,
Santa Cruz Biotechnology
). For BrdU
assay
, embryos were exposed to BrdU for 6 h before collection. For
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Reviews
Be the First to Write a Review!
BrdU (IIB5) Antibody from Santa Cruz Biotechnology, Inc.
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
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